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Figure 2. MCL-1 regulates cell surface expression levels of CXCR4 and CD44 in acute myeloid leukemia cells and leukemia-stroma interactions. (A and B) Cell surface CXCR4 and CD44 levels and migration and adhesion of vector control and MCL-1-overexpressing (MCL- 1-OE), OCI-AML3 cells (A) or vector con- trol and MCL-1-knockdown (MCL-1-KD) OCI-AML3 cells (B) to mesenchymal stromal cell (MSC). Blue bars represent the migration of OCI-AML3 cells to CXCL12 (positive control) or randomly (negative control). (C and D) Cell sur- face CXCR4 and CD44 levels and migra- tion and adhesion of OCI-AML3 cells treated with AZD5991 (C) or venetoclax (D) for 24 hours (h) to MSC. Cell surface CXCR4 and CD44 and cellular MCL-1 levels were determined by flow cytome- try. Migration was performed for 6 h and adhesion for 24 h. Experiments were performed in triplicates. Results are expressed as the mean ± standard error of the mean. IgG: immunoglobulin G; MFI: mean fluorescent intensity.
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