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Letters to the Editor
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Figure 1. Immunoglobulin G frac- tions from patients induce ERK and p38 phosphorylation with tissue factor expression. Human platelets from healthy donors were treated with immunoglobu- lin G (IgG) fractions from the 2 patients and from a healthy donor vaccinated with ChAdOx1 nCoV-19 (10 minutes for ERK and p38 activation and 4 hours for tis- sue factor [TF] analysis). Protein extracts were separated by SDS- PAGE and analyzed by western blot to investigate phosphorylat- ed ERK (A), p38 MAPK (B), and TF (C), using anti-phospho-ERK1/2, anti-phospho-p38 and anti-TF antibodies, respectively. Data are reported as mean ± standard deviation from 3 independent experiments. Statistical analysis indicated: **P<0.001 vs. untreated; §§P<0.001 vs. healthy donor IgG fraction; § P=0.01 vs. healthy donor IgG fraction; °°P<0.001 vs. pretreated with PD98059.
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