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Letters to the Editor
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Figure 2. Effect of in utero thirdhand smoke exposure on platelet physiology in the exposed male and female mice compared to clean air-exposed controls. (A) Thirdhand smoke (THS) in utero exposure enhanced platelet aggregation in the exposed male and female mice compared to clean air (CA)-exposed controls. Platelets from THS- in utero exposed and CA–exposed mice were stimulated with 1 μmol/L ADP or 0.1 U/mL thrombin before their aggregation response was measured. The experiment was repeated 3 times, with blood pooled from at least 6-8 mice each time. (B) In utero THS exposure enhanced ATP secretion in the in utero exposed male and female mice, compared to CA-exposed controls. Platelets from THS in utero exposed and CA–exposed mice were stimulated with 1 μmol/L ADP or 0.1 U/mL thrombin before their dense granule secretion (ATP secretion) was determined. Dense granules secretion responses were measured in a lumi-aggregometer. Platelets were incubated with luciferase/luciferin (12.5 μL) for the dense granule measurements. The experiment was repeated 3 times, with blood pooled from at least 6-8 mice each time. *Female and male data compared and P value calculated by two-way ANOVA while comparison within the same sex was done using Student’s t-test. Error bars represent standard deviation.
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