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The favorable storability of bThal+ RBC
Again, ROC curve analysis highlighted the potential of glucosamines to distinguish stored bThal+ RBC from their control counterparts (Online Supplementary Figure S1D). Finally, RBC from bThal+ donors were characterized by higher levels of carnosine and indole-acetate – metabolites
A
with either antioxidant activity or associated microbial metabolism, respectively, from baseline throughout stor- age, with no storage-dependent fluctuations, suggestive of differences in the circulating blood metabolome between the two groups at the time of donation (Figure 6D).
B
C
Figure 5. Distinct metabolic pathways in beta thalassemia minor donors during storage I. (A) Purine metabolism. (B) Methionine metabolism. (C) Hypoxia modulated hemoglobin loading via a sphingosine-1-phosphate effect. SAM: S-adenosyl methionine; SAH: S-adenosyl homocysteine; RibP: ribose phosphate. *P<0.05, **P<0.01, #P<0.001, †P<0.0001.
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