Page 93 - 2021_10-Haematologica-web

BLUEPRINT hematopoietic transcriptomes atlas
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Figure 5. Circular RNA expression in blood cells. (A) Bar plot of the mean number of backsplices identified in each cell type. Each bar is color-coded to indicate the number of backsplices originating in different types of genomic regions: exonic_known: the backsplice corresponds to known splice site; exonic_novel: the backsplice utilizes only one known splice site; intronic: the backsplice is internal to an annotated intron; intergenic: the backsplice junctions do not overlap any annotated exons or introns; antisense: the backsplice is antisense to annotated exons or introns. (B) Heatmap of the Spearman rank correlation (rho) between the backsplice junction counts in each sample. Lowly expressed circular (circ)RNA (having <20 reads in all samples) were excluded. (C) Heatmap of z-scores of the expression estimates for each of the differentially expressed backsplice junctions across cell types. (D) Box plot of the posterior expected log fold-change of the genes corresponding to the significantly differentially expressed circRNA, stratified by the sign of the circRNA log fold change. The posterior expected log fold changes were computed as the log fold changes conditional on differential expression multiplied by the corresponding posterior probabilities of differential expression. The center mark and lower and upper hinges of the boxplots indicate, respectively, the median, 25th and 75th percentiles. Outliers beyond 1.5 times the interquartile range from each hinge are shown. The y-axis covers the range (-3,3). Abbreviations as in Figure 1. FC: fold change.
haematologica | 2021; 106(10)
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