Page 254 - 2021_10-Haematologica-web

Letters to the Editor
considered by modern transfusion medicine. Results may have an impact on future studies regarding: the need for greater control over the process of selection, assignment, and administration of blood labile products. Moreover, they might be a call for re-evaluation and for the setting of new standards for the storage of blood products.
Ewa Szczesny-Malysiak,1 Tasnim Mohaissen,1,2 Katarzyna Bulat,1 Magdalena Kaczmarska,1 Aleksandra Wajda1,3 and Katarzyna M. Marzec1
1Jagiellonian Center for Experimental Therapeutics, Jagiellonian University; 2Faculty of Pharmacy, Jagiellonian University Medical College and 3Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Krakow, Poland
Correspondence:
KATARZYNA MARIA MARZEC - katarzyna.marzec@jcet.eu doi:10.3324/haematol.2021.278895
Received: April 12, 2021.
Accepted: June 21, 2021.
Pre-published: July 8, 2021.
Disclosures: no conflicts of interest to disclose.
Contributions: ES-M designed the study, collected flow cytometry data, analyzed and interpreted data, wrote and drafted the manuscript; TM collected the ektacytometry data, analyzed and interpreted data and wrote the manuscript; KB collected biochemical data, analyzed and interpreted data; MK collected, analyzed and interpreted AFM data; AW prepared samples for all measurements, analyzed biochemical and morphological data; KMM developed the concept
and design, provided funding, wrote and approved the final version to be published.
Acknowledgements: we would like to thank Mrs. Beata Mazurek from the Regional Center for Blood Donation and Haemotherapy in Krakow, who coordinated the donation and the preparation of pRBC used in this study.
Funding: this work was supported by the National Center for Research and Development, Poland (LIDER/13/0076/L- 8/16/NCBR/2017). The open-access publication of this article was funded by the Priority Research Area BioS under the program “Excellence Initiative – Research University” at the Jagiellonian University in Krakow.
Data sharing statement: for original data please visit http://dx.doi.org/10.17632/dwxw2wwjtm.1
Research limitation: the experimental setup was based on twelve healthy male (<30 years, n=3; 30-39 years, n=4; >40 years, n=5) and twelve female donors (<30 years, n=4; 30-39 years, n=4; >40 years, n=4) so age was evenly distributed in the range of 18-60 years for fRBC and mRBC. However, conclusions regarding comparison of specific age groups presented in the Online Supplementary Appendix should be drawn in relation to the relatively small number (n=3-5). Observed processes may be an outcome of sex-related hormones action, which were not studied in this work. Major limitations of this study could be addressed in future research.
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