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A. D'Alessandro et al.
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Figure 7. Metabolomics and post-transfusion recovery of stored red blood cells from wild-type and PCMT1-/- knockout mice. (A) Metabolomics and post-transfusion recovery of stored red blood cells (RBC) from wild-type (WT) and PCMT1-/- knockout (PCMT1 KO) mice; (B) despite no significant changes in baseline and end of stor- age post-transfusion recoveries (PTR), PCMT1 KO RBC showed significant alterations of energy and redox metabolism – as highlighted in the heat map in (C) and line plots in (D) for representative metabolites amongst the significant ones by repeated measures ANOVA as a function of storage duration.
Disclosures
The authors declare that AD and KCH are founders of Omix Technologies Inc and AD of Altis Biosciencens LLC; AD and JCZ are a consultant for Rubius Therapeutics; AD is an advisory board member for Hemanext Inc and FORMA Therapeutics Inc. All the other authors disclose no conflicts of interest relevant to this study.
Contributions
AD and JCZ designed the study; AH, JCZ performed mouse studies. AD, BB, EJM performed metabolomics analyses; MD, KCH performed proteomics analyses; AD and JCZ analyzed
data, prepared figures and wrote the first draft of the manuscript; AD and JCZ revised the manuscript. All the authors contributed to the finalization of the manuscript.
Acknowledgments
This research was supported by funds from the Boettcher Webb- Waring Investigator Award (ADA), RM1GM131968 (ADA) from the National Institute of General and Medical Sciences, and R01HL146442 (ADA), R01HL149714 (ADA), R01HL148151 (ADA, JCZ), R21HL150032 (ADA), from the National Heart, Lung, and Blood Institute.
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