Page 134 - 2021_10-Haematologica-web

L. Paschold et al.
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B
Figure 6. Clonal architecture of B lineage repertoires and lymphocyte-predominant subclones in nodular lymphocyte-predominant Hodgkin lymphoma with relapse (cohort 2). (A) Cases with identical „longCDR3“ lymphocyte-predominant (LP) rearrangement. (B) Cases with identical LP rearrangement and average-sized CDR3. IGHV/D/J maps of the global IGH repertoire are shown for each tissue sample (at initial diagnosis and relapse). Rearrangements of IGHV1-7 main groups and IGHJ1- 6 are plotted on the vertical axis. IGHD subgroups are plotted on the horizontal axis. Each dot represents one immunoglobulin rearrangement colored according to its CDR3 length and sized according to clonal fraction. A white square indicates the position of the malignant clone. A schematic overview of intraclonal diversification and evolutionary patterns of LP subclones derived from the same cell of origin are shown below. The sizes of the bubbles reflect relative clone fractions of LP sub- clones within one repertoire. Arrows indicate somatic hypermutation. Colored clones are characterized further in Table 1. NLPHL: nodular lymphocyte-predominant Hodgkin lymphoma; ID: initial diagnosis; Rel: relapse/transformation; nt: nucleotide.
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