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E. Haring et al.
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Figure 7. The graft-versus-leukemia/lyphoma effect is preserved despite tauroursodeoxycholic acid administration. (A to F) MV-4-11 and RMB-1 cells were cultured for 72 hours (h) with or without addition of tauroursodeoxycholic acid (TUDCA). One representative result from three independent experiments performed in technical triplicates is shown for panel (A) and (D). In panels (B), (C), (E) and (F) data were pooled from three independent experiments performed in technical duplicates or triplicates. P-values were calculated using the two-tailed unpaired Student’s t-test; ns: not significant. (A) Representative histograms from flow cytometric analysis of human leukocyte antigen (HLA) A, B, C expression on MV-4-11 cells. (B) Quantification of HLA A, B and C expression on MV-4-11 cells. (C) Quantitative polymerase chain reaction (PCR) analysis of the expression of the antigen presentation-related genes TAP1 and TAP2 in MV-4-11 cells with glyceraldehyde 3-phosphate dehydro- genase (GAPDH) as reference gene. (D) Representative histograms from a flow cytometric analysis of major histocompatibility complex (MHC) class I expression on RMB-1 cells. (E) Quantification of MHC class I expression on RMB-1 cells. (F) Quantitative PCR analysis of the expression of the antigen presentation-related genes Tap1 and Tap2 in RMB-1 cells with Actb as reference gene. (G) C57BL/6 CD8+ T cells were activated with allogeneic (BALB/c) dendritic cells for 72 h and treated with 500 mM TUDCA or vehicle prior to incubation with A20 lymphoma cells. The percentage of dead A20 cells after 24 h of incubation was analyzed by flow cytometry.
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haematologica | 2021; 106(8)