Page 143 - 2021_07-Haematologica-web

Hematopoietic JAK 2V617F in aortic aneurysms
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Figure 1. Hematopoietic JAK2 V617F contributes to abdominal aortic aneurysm formation. (A) Schematic diagram of the experimental design of bone marrow (BM) transplantation (BMT). BM cells from control wild-type (WT) mice or JAK2V617F mice were injected into the lethally irradiated ApoE−/− recipient mice. Five weeks after BMT, the ApoE−/− recipient mice transplanted with WT BM cells (WT-BMT mice) or JAK2V617F BM cells (JAK2V617F-BMT mice) were subjected to saline or angiotensin II (Ang II, 1900 ng/kg per min) infusion for 4 weeks. (B) Chimerism of donor cells in the peripheral leukocytes (n=11–13) and (C) blood cell counts (n=9–11) in the WT-BMT mice and JAK2V617F-BMT mice at 5 weeks after BMT. *P<0.05 versus the WT-BMT mice by the unpaired Student’s t-test. (D) Systolic blood pressure (BP) at the baseline (n=17 each), 2 weeks (n =7–10) and 4 weeks (n=7–8) after saline or Ang II infusion. (E) Representative ultrasound images of abdominal aorta under saline or Ang II infusion for 4 weeks. Scale bars, 1.0 mm. (F) Abdominal aorta diameter at the baseline (n= 33–34), 2 weeks (n=7–24) and 4 weeks (n=7–24) after saline or Ang II infusion. (G) Abdominal aortic aneurysm (AAA)-free survival rate in the WT-BMT mice and JAK2V617F-BMT mice after saline or Ang II infusion (n=7–26) by log-rank test. All data are presented as mean ± standard error of the mean. *P<0.05 vs. the corresponding saline-infused mice and †P<0.05 vs. the corresponding WT-BMT mice by one-way ANOVA with Tukey post-hoc analysis. WT, ApoE−/−: mice transplanted with bone marrow cells from wild-type mice; JAK2V617F, ApoE−/−: mice transplanted with bone marrow cells from JAK2V617F mice; Ang II: angiotensin II; WBC: white blood cell count; RBC: red blood cell count; Hb: hemoglobin concentration; Plt: platelet count.
haematologica | 2021; 106(7)
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