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Vaccine overcomes checkpoint inhibitor limitation
AB
C
Figure 7. Vaccination with the fusion vaccine leads to greater clonal T-cell diver- sity, which is further enhanced following checkpoint blockade. C57BL/6J mice were treated as described in Figure 6. Peripheral blood (PB) was then collected and assessed for T-cell diversity using targeted T-cell receptor (TCR) profiling. (A) Inverse Simpson diversity index indicating that vaccine alone and in combina- tion with checkpoint inhibitors enhance T-cell diversity (B) Refraction diversity analysis. (C) Bubble plot of the expression of the top TCR clones after vaccina- tion alone or in combination with checkpoint inhibitors. Columns represent samples and rows represent amino acid sequences of different TCR clones. The TCR clones with significant increases or decreases are represented by red and green, respectively. The fold-change of the top ten TCR clones for each sample is calculated compared to the untreated control samples.
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