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Tumor suppressor activity of RXR in AML
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Figure 4. Differentiation and apoptosis induced by all-trans retinoic acid (ATRA)/bexarotene. (A-D) Colony forming units (CFU) in methylcellulose per 2,000 MLL-AF9 leukemia cells treated as indicated and assessed in triplicate. (E) Photographs of MLL-AF9 colonies treated as indicated for 7 days. (F) Cytospin preparation of MLL-AF9 leukemia cells treated with or without ATRA and bexarotene for 96 hours (h) stained with Wright-Giemsa. (G) Cytospin preparation of MLL-AF9 leukemia cells that remains after treatment with ATRA/bexarotene and stained with Wright-Giemsa. (H) Cell division analysis of MLL-AF9 leukemia. On day 0, cells were stained with FxCycle Violet, and retained dye was assessed at indicated time points by flow cytometry. (I) Annexin V staining of MLL-AF9 leukemia cells after 24, 48, 72, 96, and 120 h of ATRA and bexarotene treatment in triplicate. (J-L) Relative activity of caspases 3/7, 9, and 8 in MLL-AF9 leukemia cells after 48 h of ATRA and bexarotene treatment in triplicate. *P<0.05, **P<0.01, ***P<0.001, t-test with Welch’s correction relative to control.
haematologica | 2021; 106(4)
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