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Novel PBD inhibit NF-κB in hematologic cancers
A
B
Figure 4. PBD show marked inhibitory effects on both canonical and non-canonical NF-κB subunits. JJN3 and U266 cells were treated with DC-1-92, DC-1-170 and DC-1-192 for 4 h. Nuclear extracts were then generated from these samples and the amounts of p65, p50, p52 and Rel B were quantified and expressed as relative fold-change as a function of the untreated controls. (A) JJN3 cells showed significant reductions in nuclear expression of p65, p50 and RelB NF-κB subunits but no change in p52 following exposure to DC-1-92, DC-1-170 and DC-1-192. (B) In contrast, U266 cells showed significant reductions in nuclear expression of all four NF- κB subunits. All experiments were performed in triplicate. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. ns: not statistically significant differences.
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