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Letters to the Editor
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Figure 2. Sensitization to cytarabine (Ara-C) by inhibition of glucose uptake. (A) Immunofluorescence of GLUT1 expression (magenta) in THP-1 cells and KG-1 cells. Nucleus stained with DAPI. 60x magnification, scale bars=30 mm. (B) Western blot of THP-1 and KG-1 whole lysates; GAPDH as the loading control. (C and D) Relative inhibition of cell viability in THP-1 (magenta) and KG-1 (blue) cells using Ara-C and (C) PGL-13 or (D) PGL-14, alone or in combination at IC25. Co- treatment effects were destined synergistic or additive/no effect as determined by the Bliss independence model through calculation of the combination index (CI). SSynergy (CI<1) and is based on CI-values (C) 0.64 and (D) 0.78. (E and F) Relative inhibition of cell viability in THP-1 cells using Ara-C, Brequinar (BQR) and (E) PGL-13 or (F) PGL-14, alone or in combination. Combination of PGL with Ara-C gave values (E) CI=0.76 and (F) CI=0.90. All co-treatment values show mean+standard deviation normalized to a DMSO control, n=3-5. Dotted lines show theoretical IC25 values.
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