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J.V. Neves et al.
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Figure 1. Hematologic parameters, bone marrow (BM) erythroid populations, serum parameters and tissue iron content in BALB/c mice. Blood, serum and tissue samples were collected at 1, 4, 7, 14 and 21 days post infection with T.b. brucei. Hematologic parameters were blindly determined by a certified laboratory and BM cells were isolated, stained with antibodies for erythroid populations and analyzed by flow cytometry. Serum parameters were blindly determined by a certified lab- oratory and iron content in the liver and spleen was measured by the bathophenanthroline method. (A) Red blood cells (RBC), (B) reticulocytes (RET), (C) hematocrit (HCT), (D) hemoglobin (Hb), (E) mean corpuscular volume (MCV), (F) total erythroid cells (Ter119+), (G) pro-erythroblasts (Pro-E), (H) basoerythroblasts (Baso-E), (I) polychromatic erythroblasts (Poly-E), (J) serum iron [Fe], (K) transferrin saturation (TSAT), (L) total iron binding capacity (TIBC), (M) serum ferritin, (N) total liver iron [Fe], (O) total spleen iron [Fe]. Values are represented as mean±standard deviation (n=5). *P<0.05, **P<0.01, and ***P<0.001 were considered statistically sig- nificant.
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haematologica | 2021; 106(3)