Page 138 - 2021_03-Haematologica-web

K. Jimenez et al.
AB
CD
E
FG
Figure 1. Iron deficiency causes thrombocytosis, which is reversed by iron replacement therapy in a dose-dependent fashion. (A) Experimental design. Rats were fed an iron-deficient diet (Def) for 6 weeks, and given three injections of ferric carboxymaltose at the doses of 5, 10, or 20 mg/kg body weight (BW, +Fe5, +Fe10, +Fe20, respectively) or placebo (4 mL/kg BW 0.9% NaCl). Animals fed a control diet and given injections of placebo formed the control group (Con). Hematologic parameters were measured weekly from 3 to 6 weeks after the start of the experiment (n=4 per group). (B). Changes in hemoglobin and mean corpuscular volume from 3 to 6 weeks in the different groups of animals. (C) Values of mean corpuscular hemoglobin, hematocrit and platelet count over the course of the experiment. (D) Platelet counts versus hemoglobin concentration at 6 weeks. (E) Representative images of liver and spleen histological staining for iron (Prussian blue stain). Red arrows mark positively stained cells. Red asterisks (*) mark central veins. (F) Relative expression of hepcidin as determined by real-time quantitative polymerase chain reaction analysis of liver isolates. Values are normalized to those in Con animals, with Hprt1 as the endogenous control. (n=3-4 per group). (G) Blood loss and bleeding time measured during the tail bleeding assay (n=3-4 per group). Error bars: mean ± standard deviation. FCM: ferric carboxymaltose, Hb: hemoglobin, MCV: mean corpuscular volume, MCH: mean corpuscular hemoglobin, HCT: hematocrit, PLT: platelet count
784
haematologica | 2021; 106(3)