Page 124 - 2020_11-Haematologica-web

Y. Zhou et al.
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Collagen (mg/mL) Thrombin (U/mL)
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Figure 2. Migfilin-/- platelets display impaired aggregation responses due to defective dense granule secretion. (A) Platelets were stimulated with collagen, thrombin, U46619, and ADP (in the presence of fibrinogen). Aggregation and ATP release was assessed with a Chrono-log lumiaggregometer under stirring at 1,200 rpm. Traces are representative of at least three independent experiments. Results are expressed as mean ± standard error of the mean (SEM) from at least four independent experiments. Statistical significance was evaluated with paired Student t test. (*P<0.05, **P<0.01). (B) Aggregation of washed WT or migfilin-/- platelets stimulated with collagen (0.4 mg/mL) or thrombin (0.018 U/mL) in the presence of vehicle (left panel), or apyrase (0.25 U/mL) (middle panel), or a low concentration of ADP (1 μM) (right panel). Percentage of platelet aggregation from at least four independent experiments is depicted on the right, and the results are shown as mean ± SEM (*P<0.05, **P<0.01, ***P<0.001, ns: no significant difference, paired Student t test).
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