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I.A. de la Rosa et al.
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Figure 6. miR-126, miR-148a and miR-223 regulate the expression of specific mRNA targets involved in inflammation, migration and cell survival on neutrophils.
DICER downregulation induces an inflammatory profile in neutrophils. (A) Overexpression of miR-126 decreased the expression of VEGF in RA active neutrophils. (B) Overexpression of miR-148a decreased the expression of TNF-a in RA active neutrophils. (C) Overexpression of miR-223 decreased the expression of IL-8 and Il-1β and increased VEGF in RA active neutrophils. (D) DICER expression levels after lentivirus transfection by PCR and Western blot. (E) Expression levels of miRNA in DICER downregulated neutrophils. (G) Protesome array of cytokines and chemokines in HL60 neutrophil-like cells after downregulation of DICER by lentiviral trans- fection. MiRNA: microRNA; RA: rheumatoid arthritis; VEGF-A: vascular endothelial growth factor A; TNF-a: tumor necrosis factor-alpha; IL-8: interleukin-8; IL-1β: ιnterleukin-1 beta; CCL1: chemokine (C-C motif) ligand 1; CCL2: chemokine (C-C motif) ligand 2; MIP-1a/1β: macrophage inflammatory proteins alpha and beta; CCL5: chemokine (C-C motif) ligand 5; IL-1ra: interleukin 1 receptor antagonist; IL-6: interleukin-6; IL-13: interleukin-13; IL-16: interleukin-16; IL-17a: interleukin-17a; MIP: macrophage migration inhibitory factor; PAI-1: plasminogen activator inhibitor-1; Scr: scrambled; L-Scr: lentivirus-scrambled; L-DICER: lentivirus-DICER. Data are presented as mean ± standard error of the mean (SEM) of two independent experiments; a: significant differences P<0.05.
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