Page 78 - 2020_09-Haematologica-web

I.A. de la Rosa et al.
A
BC
DE
Figure 4. Reduced levels of miRNA in rheumatoid arthritis neutrophils are related to ACPA. (A) Correlation studies of reduced miRNA and DICER expression levels in neutrophils from peripheral blood with clinical parameters such as, ACPA levels, DAS28, CRP and inflammatory markers including TNF-a and IL-6 serum cytokine levels. (B) In vitro effect of enriched IgG-ACPA in miRNA expression in healthy neutrophils. (C) In vitro effect of enriched IgG-ACPA in the expression of genes related to the miRNA biogenesis in healthy neutrophils. (D) In vitro effect of enriched IgG-ACPA in the mRNA expression of potential mRNA targets of the validated miRNA. (E) Proteome profile of chemokines and cytokines in neutrophils treated with IgG-NHS and enriched IgG-ACPA. ACPA: antibodies to citrullinated protein antigens; IgG- NHS: immunoglobulin G from normal human serum; miR: microRNA; HD: healthy donor; DAS28: disease activity score 28; CRP: C-reactive protein; TNF-a: tumor necrosis factor-alpha; IL-6: interleukin-6; AGO-1: argonaute-1; AGO-2: argonaute-2; XPO-5: exportin-5; VEDF-A: vascular endothelial growth factor A; IL-1β: interleukin- 1 beta; IL-8: interleukin-8; IL-6R: interleukin-6 receptor; STAT-3: signal transducer and activator of transcription 3; AKT: protein kinase B; CCL1: chemokine (C-C motif) ligand 1; CCL2: chemokine (C-C motif) ligand 2; MIP-1a/1β: macrophage inflammatory protein 1 alpha/1 beta; CCL5: chemokine (C-C motif) ligand 5; CD40 ligand: cluster differentiation 40 ligand; CXCL-1: chemokine (C-X-C motif) ligand 1; CXCL12: chemokine (C-X-C motif) ligand 12; G-CSF: granulocyte colony-stimulating factor; GM-CSF: granulocyte-macrophage colony-stimulating factor; IFN-g: interferon gamma; IL-1a: interleukin-1 alpha; PAI-1: plasminogen activator inhibitor-type 1. Data are presented as mean ± standard error of the mean (SEM) (n= 5); a: significant differences P<0.05.
2256
haematologica | 2020; 105(9)