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ASLAN003 for differentiation of AML
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Figure 4. ASLAN003-induced apoptosis involves both mitochondrial and death receptor pathways. (A) Whole-cell lysates or cytosolic fractions from KG- 1 and MOLM-14 cells treated with dimethylsulfox- ide (DMSO), 0.5 μM or 1 μM ASLAN003 for 48 h were used to measure apoptosis-related proteins by western blot analyses. (B) THP-1, MOLM-14 and KG- 1 cells were exposed to 1 μM or 2 μM ASLAN003 or DMSO as the control for 48 h and then stained with JC-10 and analyzed by flow cytometry for quantifica- tion of intrinsic mitochondrial membrane potential (MMP). Representative FACS plots are shown. The bars in the bar chart represent the means of the increased depolarization of MMP of the three cell lines after exposure to ASLAN003 in two independ- ent experiments, the error bars denote the stan- dard deviation.
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