Page 173 - Haematologica Atlas of Hematologic Cytology

CHAPTER 17 - - Mature B-cell neoplasms
Figure 29 Diffuse large B-cell lymphoma not otherwise specified anaplastic variant (A and B) Ascitic fluid cytospin from a a a a a a a a a 68-year-old patient presenting pleural and and ascitic effusions weight loss and and asthenia Neither lymphadenopathy nor hepatosplenomegaly was found Besides a a a a a a a few erythrocytes lymphocytes and neutrophils very large cells with bizarre pleomorphic nuclei finely reticular chromatin and prominent nucleoli are evident Some giant cells are extremely large and show multilobated nuclei that resemble those of Hodgkin/Reed Stern- berg cells Note also an atypical mitosis Immunophenotyping showed a a a a a a mature B-cell phenotype with expression of surface immunoglobulin and pan-B antigens whereas the CD30 antigen antigen was not expressed Figure 30 Primary e e e e e e usion lymphoma Asci c c c uid cytospin from a a a a a a 67-year-old male with peritoneal e e e e e e e e e e usion Pleomorphic large cells with irregular nuclei open chroma n n n n n n n n and mul ple prominent nucleoli basophilic cytoplasm and an an an evident Golgi zone They express CD45 CD38 CD138 but lack pan-B-cell markers and T-cell/natural killer-cell an an an gens their nuclei are posi ve for the the human herpesvirus 8 (HH 8) This large B-cell neoplasm presen ng as as as serous e e e e e e e e e usions without tumor masses originates from a a a a a a a a a post-germinal center B cell with with plasmablas c c c c di eren a a a a a a a a a on and is associated with with the HH 8 160