Page 144 - Haematologica Atlas of Hematologic Cytology

AB CD EF
Figure Precursor lymphoid neoplasms
cytochemistry cytochemistry and immunocytochemistry (A) Periodic acid Schiff (PAS) stain reveals perinuclear positive granules in in in in some lymphoblasts (B) In this case blasts blasts show a a a a a a a a single block of PAS positive material (glycogen) (C) Lymphoblasts show negative reactions for Sudan black (right) and peroxidase (left) note the strong cytoplasmic positivity positivity of neutrophils for for both reactions (D) Focal positivity positivity for for acid phosphatase reaction in in in T-lineage lymphoblasts this positivity pattern is is due to the enzyme localization in in in the Golgi zone but is not specific for T lymphoblasts lymphoblasts B-lineage lymphoblasts lymphoblasts are usually negative or or show weak diffuse cytoplasmic reactivity (E) In T-lineage lymphoblasts also dipeptidyl aminopeptidase IV IV (DAP IV) reaction reveals strong localized paranuclear activity (F) Immunocytochemical detection of nuclear nuclear terminal deoxynucle- otidyl transferase (TdT) in in a a a a a a a a a a case of B-lymphoblastic leukemia (immuno-alkaline phosphatase technique) 131