Page 114 - Haematologica Atlas of Hematologic Cytology

AB
CD
EF
Figure 1 1 1 1 1 1 cute myeloid leu emia with t( 1)( 1) 1) RUNX1-RUNX1T1 Bone marrow smear from a a a a a a a a a a a a a a a a a a a 58-year-old woman admi ed ed ed to to to hospital because of fa gue pallor and and cutaneous purpura Physical examina on on revealed hepato- megaly A A blood blood count count showed severe anemia thrombocytopenia and and and normal white blood blood cell cell count count with with with rare blasts (A) Granuloblas c c c c c c c c c c c c c c c c c hyperplasia without hiatus and and and increased blast blast cell cell percentage (40%) Blasts are are are rather large with with with with eccentric o o o o o o o o o o o o o en en en en en en indented or cleaved nucleus basophilic cytoplasm with with with a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a a clear clear clear area at at at at the the nuclear nuclear indenta on on and and so- me me mes many many azurophilic granules (B) Maturing myeloid cells are are characterized by evident asynchronous nuclear:cyto- plasmic plasmic matura on on on with with with open chroma n n n n n n n n n n n n n n n n n n n n n n n n n n and prominent nucleoli also in in in in the presence of many many secondary cytoplasmic granules (C) (Center) Blast cell with with with large eccentric indented nucleus abundant cytoplasm cytoplasm with with with peripheral basophilia prominent Golgi zone and and an an an an an an Auer Auer rod rod (D) (Right) Neutrophil showing a a a a a a a a a a a a a a a a a a a a a a a a very long slender Auer Auer rod rod demonstra ng ng ng its deriva on on on on on on on from a a a a a a a a a a a a a a a a a a a a a leukemic blast blast Note also the neutrophil neutrophil degranula on on on on on on on and and abnormal nuclear segmenta on on on on on on on (E) Pe- riodic acid Schi (PAS) staining is nega ve in in in in in blast blast cells cells some some neutrophils neutrophils show show less strong cytoplasmic posi posi vity vity than normal neutrophils neutrophils (F) Blast cells cells show show Sudan black posi posi vity vity some some mes in in in in in a a a a a a a a a a a a a a a a restricted area at the nuclear indenta on on 101