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V. Giudice et al.
CD8+CD57+ T-cell expansion. By χ2 test, transfusion histo- ry did not correlate to CD8+CD57+ expansion (P=0.255).
Total CD8+ cell TCR repertoires are polyclonal in healthy subjects
Deep sequencing of TCR repertoire was performed in CD4+ and CD8+ populations sorted from 9 healthy donors. The average depth of sequencing was 10,790,646±4,050,138
in CD4+ T cells, and 10,961,961±3,879,596 in CD8+ popula- tions. The mean frequency of the immunodominant clone was 4.1±4.1% in CD4+ cells and 17.3±16.9% in CD8+ cells. By plotting the number of reads of each Vβ and Jβ matching,17 a “citylike” landscape was described for total CD4+ and CD8+ T-cell populations (Figure 3A and Online Supplementary Figure S7A), because of the presence of a more homogenous distribution of TCR rearrangement fre-
A
B
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Figure 4. The TCR repertoire by deep sequencing analysis from total CD8+ cells in severe aplastic anemia (SAA) patients with CD8+CD57+ cell expansion. In contrast to healthy CD8+ profiles, most SAA patients (AA) displayed oligoclonal features in a TRBV/TRBJ rearrangement plot (A) and CDR3 size and DJ length profiles (B). CD4+ and CD8+ profiles are shown for each SAA patient. In AA1 and AA6, only CD8+ cells were sufficient for deep sequencing.
haematologica | 2018; 103(5)