Page 182 - 2020_08-Haematologica-web

V.M. Smith et al.
AB
CD
Figure 5. BH3-mimetic-induced cell death is partial- ly dependent on BIM. (A-C) Depletion of BIM was achieved using a combination of two distinct short- interfering (si)RNA oligonucleotides (siBIM#1 and siBIM#2). Data on individual BIM siRNA are provided in Online Supplementary Figure S6. Upon transfec- tion, cells were treated with the indicated concentra- tions of ABT-199 (A) in RIVA or U2932 cells, upon treatment with A1331852 (B) in RCK8 or SUDHL8 cells, or upon treatment with S63845 (C) in SUDHL10 or U2946 cells for 4 or 6 h and phos- phatidylserine (PS) exposure was analyzed by stain- ing with annexin V-FITC and flow cytometry. Non-tar- geting siRNA (siCtrl) was used as a control. The mean and standard deviation are shown (n=3-4). (D). Knockdown efficiency was controlled by western blotting 48 h after transfection. *P<0.05;**P<0.01; ***P<0.001.
2158
haematologica | 2020; 105(8)