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Minihepcidins to treat ß-thalassemia major mice
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Figure 5. Serum and tissues iron analysis showed improvements in Hbbth1/th2-BMC mice after treatment with minihepcidin. In a comparison of animals treated with vehicle (V) or high-dose hepcidin (MH_H), (A) serum erythroferrone (ERFE) levels were significantly different, (B) serum hepcidin (HAMP) was decreased, (C) while transferrin saturation did not show significant differences. (D) Serum iron was significantly reduced after MH_H administration. As a result of a decreased erythroid iron uptake, total organ iron content was reduced in the (E) liver and (F) spleen, but not in the kidney (not shown). Bars represent the standard deviation. ****P≤0.001, **P≤0.01; *P≤0.05.
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Figure 6. Minihepcidin and blood trans- fusion improved hematologic parame- ters and reduced serum erythropoietin in Hbbth1/th2-BMC mice. Administration of minihepcidin resulted in (A) increased red blood cell (RBC) count and (B) hemo- globin (Hb) levels, while (C) reticulocytes (RETIC) numbers were reduced. (D) Serum erythropoietin (EPO) concentra- tion did not show statistical differences. Bars represent standard deviation. ****P≤0.001, **P≤0.01. WT: wildtype; FLC: fetal liver cells; V: vehicle; MH_H: high-dose minihepcidin; Bl.Tr: blood transfusion.
haematologica | 2020; 105(7)
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