Page 288 - Haematologica - Vol. 105 n. 6 - June 2020
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M. Salzmann et al.
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E after diphtheria toxin (DT) treat- ment of wild-type (WT) and iDTRPlt mice, one group of iDTRPlt mice received platelet transfu- sion, followed by thioglycollate injection. After three days peri- toneal macrophages were iso- lated and quantified. (B) Recruited CD45+ cells and (C) CD45+ F4/80+ cells per mouse (n=5-9, mean ± standard error of the mean [SEM]). (D) Representative blots of peri- toneal lavage cells of WT mice, iDTRPlt mice and iDTRPlt mice with platelet transfusion. (E) Graphical overview of FeCl3 induced thrombus formation experiments. Seven days after DT treatment of iDTRPlt mice, one group received Nbeal2+/+ and the other Nbeal2-/- platelets, followed by induction of a thrombus in mesenteric arterioles by topic application of FeCl3. (F) Time to stable ves- sel occlusion. Each symbol rep- resents a mesenteric arteriole. (G) Representative images of thrombus formation in arteri-
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Figure 6. Platelet function and phenotype remains intact after transfusion. (A) Time plan of macrophage recruitment after sterile peritonitis. Seven days
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haematologica | 2020; 105(6)
