Page 193 - Haematologica May 2020

miR-146b in T-LGL Leukemia patients
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Figure 1. miRNome of T-LGLL patients correlates with STAT3 phosphoryla- tion. Peripheral blood CD57+ T cells from T large granular lymphocyte leukemia (T-LGLL) patients (n=6) and healthy donors (HD, n=3) were collected. (A) Heatmap representation of the 756 miRNAs analyzed using the TaqMan microRNA array as described in the Methods. Results are expressed as arbi- trary units (au) using U6 as reference control. (B) Whole-cell extracts (250x103 cells) purified from the same cells as in (A) were loaded on gels and analyzed for STAT3-YP and total STAT3 expression level. Quantification of the relative normalized STAT3-YP protein levels are reported as arbitrary units (au) below the Western blot. Mean ± standard error of the mean (SEM) is shown, *P<0.05 by unpaired t-test. (C) Absolute neutrophil counts in each CD8 and CD4 T-LGLL patient (circle) and HD (triangle). Mean ± SEM is shown. **P<0.01 by unpaired t-test. Patients analyzed in each panel are specified in the Online Supplementary Table S5.
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