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classes of metabolites. This analysis revealed that numer- ous constituents of cell membranes were less abundant in OMP52M51-resistant versus control PDTALL19 samples, including members of glycerophospholipids, sphin- gomyelins and ceramides families, as well as oxysterols and one cholesterol ester. Only few metabolites in these
A
classes were enriched in OMP52M51-resistant cells (Figure 4A). Quantification of the cellular contents of lipid droplets by flow cytometry confirmed a significant reduc- tion of lipid storage in OMP52M51-resistant cells com- pared to controls (Online Supplementary Figure S11). Moreover, MS/MS analysis discovered an imbalance
B
Figure 3. Transcriptome analysis of OMP52M51-resistant PDTALL19 cells. (A) Heatmap of genes modulated comparing OMP52M51 resistant and control cells (4- 5 samples/group). Red and blue colors indicate higher and lower expression levels, respectively. The columns represent individual samples (B) top: Gene set enrich- ment analysis (GSEA) plots of four enrichment sets down-regulated in resistant cells. Bottom: Heatmaps and lists of the top 25 differentially expressed genes of the corresponding plots reported above.
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haematologica | 2020; 105(5)