Page 141 - Haematologica April 2020

Clofazimine inhibits chronic myeloid leukemia
A
F
B
C
D
G
H
EI
JKL
Figure 8. Effects of clofazimine and clofazamine + imatinib on K562 xenografts. (A) Photographs of mice with tumor, tumor volumes on day 1 (d1) and day 13 (d13) are given. The red ‘O’ represents an outlier based on d1 tumor volume (>150 mm3). (B) Relative tumor volume (day wise), left panel; all data included, right panel; minus the outlier (as shown in A). (C) Photographs of tumors. (D) Tumor weight, left panel; all data included (outlier marked in red); right panel: minus outlier. (E) Hematoxylin & eosin staining of tumor sections (red arrowhead: mitotic cells; red arrow: vasculature; yellow arrow: myeloblasts; green arrow: pyknotic nuclei; cyan arrow; karyorrhexis; black arrow: degenerating cells). (F) Ki67 staining. (G) Number of Ki67-positive nuclei. (H) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. (I) Number of TUNEL signals/ number of nuclei in a field. (E-I) Eighteen fields/group (6 animals/group). (J-L) Body weight (J), body weight normalized liver weight (K), and body weight normalized spleen weight. (J-L) Vehicle; n=7, all treatment groups, n=6 per group. Microscopy was performed with a Leica DMI6000B microscope (Leica) and the findings were quantified with Image J software. (F, H) For counting, intensity of all images was enhanced in Microsoft office picture manager at a mid-tone setting of 80. Cropped images with uniformly increased brightness are given here for clarity; corresponding full-size images are presented in Online Supplementary Figure S13A, B. *,#,$P<0.05, **P<0.01, ***P<0.001. (B) Two-way analysis of variance (ANOVA), (J-L) One-way ANOVA followed by the Bonferroni post-test, (D) As indicated. (G, I) Kruskal-Wallis test followed by the Dunn test.
haematologica | 2020; 105(4)
983