Page 74 - Haematologica March 2020

G. Hernandez et al.
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Figure 2. Myeloid expansion in the bone marrow of mice with collagen-induced arthritis. (A) Experimental design and numbers of colony-forming units (CFU)-granu- locyte-macrophage (GM), CFU-burst (CFU-B) (n=4 per group) and CFU-erythroid (CFU-E) (n=8/group) in unfractionated bone marrow cells from control mice (Ctrl) and mice with collagen-induced arthritis (CIA). (B-I) Numbers of the indicated populations (see Online Supplementary Figure S1 for FACS gating and surface marker def- initions) expressed as number per million cells. (H) Representative FACS plots showing the gating strategy for identifying the CD41+ fraction of hematopoietic stem cells (HSC) (n=6 Ctrl and 11 CIA). The data were compiled from three independent experiments. (J) Experimental design and (K, L) Fluidigm gene expression analysis of HSC from Ctrl and CIA mice showing (K) myeloid and (L) megakaryocyte (Mk), erythroid (E) and lymphoid (Ly) lineage genes. The data are presented as log10 fold expression in CIA HSC versus Ctrl HSC. Ct values were normalized to Actb (n=8-16 per group). Data are compiled from two independent experiments. *P<0.05; **P<0.01 ***P<0.001, as determined by the Mann-Whitney U-test. BM: bone marrow; Pre GM: precursor granulocyte-macrophage; GMP: granulocyte-macrophage progenitors, CLP; common lymphoid progenitors; Pre MkE: precursor megakaryocyte/erythroid; MkP: megakaryocyte progenitors; MPP: multipotent progenitors; qRT- PCR: real-time quantitative polymerase chain reaction.
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haematologica | 2020; 105(3)