Page 168 - Haematologica March 2020

M. Xu et al.
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Figure 4. The abnormal myeloid cell expansion in Tg(hsp70:p210BCR/ABL1) fish caused by proliferation and apoptosis perturbation. Lcp1 and BrdU antibody immuno- fluorescence double staining indicates a significant increase in the myeloid cells proliferation in the PBI region of 3 dpf HS Tg(hsp70:p210BCR/ABL1) larvae (n=28) com- pared with the wild-type (WT) controls (n=18) (A and B) and kidney marrow (KM) Lcp1+ cells in 1-year Tg(hsp70:p210BCR/ABL1) adults (n=3) compared with the WT con- trols (n=3) (E and F). Lcp1 antibody and transferase dUTP nick end labeling (TUNEL) assay co-staining were used to detect the apoptosis. The number of Lcp1+ cells in the PBI region (C and D)/KM (G and H) in 3 dpf/1-year HS Tg(hsp70:p210BCR/ABL1) (n=27/n=4) significantly decreased compared with WT (n=19/n=5). Arrows indi- cate Lcp1BrdU/Lcp1TUNEL double-positive cells. Original magnification x100 (A, C, E, G). Percentage of the PBI region and KM localized Lcp1+ myeloid cells that incorporate BrdU/TUNEL in Lcp1+ myeloid cells in 3 dpf or 1-year HS WT and Tg(hsp70:p210BCR/ABL1) fish. Student t-test; mean±Standard Error of Mean; *P<0.05; ****P<0.0001.
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