Page 106 - 2019_12-Haematologica-web

M. Wu et al.
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Figure 1. Suppression of DOCK2 expression in MV4;11 cells resulted in differential response to ara-C and 5-fluorouracil treatment. (A) Stable knockdown (KD) of DOCK2 expression increased the fraction of cells in apoptosis upon treatment with ara-C and decreased the fraction of cells in apoptosis upon treatment with 5-flu- orouracil (5-FU) in MV4;11 cells, but not REH cells. Cells were treated for 72 h. The concentration of ara-C and 5-FU used for each cell line was the IC50 as determined by MTT assay. (B) DOCK2 KD resulted in increased cell death upon treatment with ara-C and decreased cell death upon treatment with 5-FU in MV4;11 cells, but not REH cells. (C) DOCK2 KD MV4;11 cells exhibited greater impairment in cell cycling after ara-C treatment, and less disruption of cell cycling after 5-FU treatment, compared to control MV4;11 cells. A bromodeoxyuridine (BrdU) incorporation assay revealed the cell cycle status of MV4;11 cells at 0, 2 and 14 h after treatment with ara-C (3 μM) or 5-FU (0.5 μM). At each time point, cells were pulse-labeled with 10 μM BrdU for 30 min before harvesting. The percentage of cells in each phase of the cell cycle is indicated in the bottom panel. **P<0.01; ***P<0.001; ****P<0.0001. C: cells expressing control short hairpin (sh)RNA; KD: cells expressing shRNA against DOCK2.
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haematologica | 2019; 104(12)