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J. Liu et al.
level in the spleen was reduced by more than 20% in mice upon treatment with compound 93 for 4 weeks (P<0.001). In the meantime, the serum erythropoietin level remained unchanged upon treatment with com- pound 93 (Online Supplementary Figure S22). Our data
indicated that all three compounds significantly improved ineffective erythropoiesis in the bone marrow and spleen. Considered together, these results demonstrated that our compounds effectively relieved anemia in Hbbth3/+ mice by correcting ineffective erythropoiesis.
ABC
DE
F
Figure 7. Compounds 93, 156 and 165 alleviated iron overload in Hbbth3/+ mice. (A) Serum hepcidin, (B) serum iron, (C) serum ferritin (D) hepatic iron, (E) splenic iron and (F) 3'-diaminobenzidine-enhanced Perls iron staining of liver sections (in brown) and Perls Prussion staining of spleen sections (in blue, indicated by arrows) after administration of compounds 93, 156 and 165 to Hbbth3/+ mice at a dose of 10 mg/kg body weight every other day for 2 weeks (n=4-6). Original magnification, ×400. *P<0.05, relative to untreated, control (Ctrl) mice.
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