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Figure 2. CRBN mRNA levels of multiple myeloma (MM) cells and their correlation with sensitivity to ARV 825. (A) CRBN expression in tumor cell lines (Broad Cancer Cell Line Encyclopedia). (B) Positive correlation [correlation coefficient (R)=0.6] between CRBN mRNA expression in eight parental MM cell lines (2-D(Ct) x 105), and their sensitivity to ARV 825, n=3; P<0.001) (Left). Relative CRBN mRNA expression of isogenic lenalidomide resistant (MM1S res and KMS11 res) and isogenic wild- type (MM1S and KMS11) myeloma cells, and comparison of their sensitivity to increasing concentrations of ARV 825 [growth inhibition (MTT) of the cells]. (Right, top) MM1S and MM1S res cells and (right, bottom) shows KMS11 and KMS11 res cells, and their IC50s to ARV 825. (C and D) Levels of CRBN mRNA expression (left) and protein (right, top) from KMS11 and KMS28BM cells, and their viability in ARV 825 (right, bottom) after (C) overexpression and (D) shRNA silencing. OE: overexpression. *P≤0.01; **P≤0.001; ***P≤0.0001.
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