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K. Van Roosbroeck et al.
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Figure 6. microRNA analysis after transfer of chronic lymphocytic leukemia B cells into NSG mice and in vitro functional studies. (A) Schematic representation of the chronic lymphocytic leukemia (CLL) mouse model. (B) miR-150 and miR-26a are significantly downregulated in post-transfer peripheral blood mononuclear cells in mice compared with pre-transfer CLL samples. (C) Two different CLL-specific cell lines – MEC1 and HG3, and one diffuse large B-cell lymphoma cell line – HB – were used for functional assays (upper panel). Proliferation of MEC1, HG3 and HB cells was assessed by a CellTiter 96 AQueous One Solution assay. The cells were transfected with scrambled mimic or miR-21 mimic for 24, 48 and 72 h (middle panel). Representative images and graphs representing at least three independent experiments of the soft agar colony-formation assay for miR-21 mimic and control mimic transfected MEC1, HG3 and HB cells (lower panel). *P<0.05; **P<0.01; ***P<0.001. For additional Information, see Online Supplementary Figure S6.
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haematologica | 2019; 104(5)