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miRNA in Richter syndrome
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Figure 5. microRNA network reprogramming during Richter transformation. (A) The 25-miRNA quantitative reverse transcription polymerase chain reaction (qRT- PCR) expression network in the four groups analyzed. (B) Analysis of the number of edges (left panel) and connectivity (middle and right panels). (C) Ingenuity canon- ical pathway analysis – top 20 signaling pathways for validated targets of miR-21, miR-146b, miR-150 and miR-181b and (D) for the miRNA hubs specific for Richter transformation (miR-191, miR-17 and miR-29c). The heatmap shows the most significantly involved pathways, considering validated targets of all miRNA (last col- umn). White to purple boxes represent log(P-value) with the darkest boxes being the statistically most significant. P-values for targets of each miRNA separately are given in the first four columns. Due to the large number of targets, to increase the confidence of the data, we considered a P<0.01 as an inclusion criterion. For addi- tional Information, see Online Supplementary Figure S5.
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