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P.P. Kulkarni et al.
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Figure 3. Decreased metabolic flux through the pentose phosphate pathway impairs platelet responsiveness to agonist stimulation. (A and B) PAC-1 binding in platelets treated with different reagents as indicated. (C and D) Binding of fluorescent fibrinogen to platelets treated with different reagents as indicated. (E and G) Platelet aggregation induced by thrombin (0.2 U/mL) and collagen (2 mg/mL), respectively in the presence of vehicle (control), DCA (20 mM), DASA (200 mM) or DHEA (200 mM) (tracings 1, 2, 3 and 4 respectively). (F and H) Scatter dot plots showing platelet aggregation. (I and J) Fluorescent-labeled annexin V binding to platelets treated with various reagents as indicated. Data are presented as the mean ± standard error of mean. Each dot represents an independent observation. (*P<0.05 as compared to resting platelets; #P<0.05 as compared to thrombin-stimulated platelets). Apo: apocynin; DASA: diarylsulfonamide; DCA: dichloroacetate; DHEA: dehydroepiandrosterone sulphate; RP: resting platelets; Thr: thrombin
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haematologica | 2019; 104(4)