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Figure 7. TIRAP p.R81C enhances NF-kB activity and protects against stress-induced apoptosis. 293T cells were transfected with TIRAP WT-GFP, TIRAP p.R81C-GFP or empty vector (EV)-GFP plasmids. Twenty-four hours (h) post transfection, 293T cells were FACS purified for GFP-positive cells. (A) Heatmap showing hierarchical clustering of mRNA lev- els of six selected genes involved in NF-kB pathway, cell survival and proliferation within GFP-positive 293T cells. Bar chart showing the log2 fold difference of gene expression in transfected 293T cells (TIRAP p.R81C or WT vs. EV) of a representative experiment. (B) FACS purified GFP-positive 293T were cultured in starvation medium for 48 h, and cell viability was assessed by flow cytometry. Bar chart (mean±Standard Error of Mean) show percentage of viable cells (AnnexinV– and Viability dye–) of 2 independent experi- ments performed in replicates. One-way ANOVA with Bonferroni post hoc test was used, ***P<0.001.
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