Page 59 - 2019_01-Haematologica-web

Stromal cells need macrophage iron
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Figure 1. Transient alopecia and anemia are present in Fpn1fl/flLysCre+/- mice. (A) Hemoglobin (Hb) levels and hematocrit (Hct) in 3- to 6-week (w) old mice (mean ± SEM of 50 mice for each group; ***P<0.0001, **P<0.001). The histogram at the bottom shows the percentage alopecia at different time-points (mean ± SEM of 50 mice for each group; ***P<0.0001, **P<0.001 versus Fpn1fl/flLysCre-/-). (B) Top: Hb levels, Hct, serum iron and transferrin saturation (TS) in 3-, 6-, 12-, and 18- week old mice (mean ± SEM of 50 mice for each group; ***P<0.0001, **P<0.001, *P<0.01). Bottom: hepcidin (HAMP) expression in the liver (solid bars) and skin (striped bars) and spleen erythroferrone (Fam132b) mRNA levels of 3-, 6-, 12-, and 18-week old mice. mRNA levels were measured by quantitative real time poly- merase chain reaction and normalized to the housekeeping gene 18S RNA. Data are presented as mean ± SEM of 10 mice for each group. (C) Representative appear- ance of 3-week old Fpn1fl/flLysCre-/- (left) and Fpn1fl/flLysCre+/- (right) mice and representative histology (dorsal area) of the same mice. Magnification 10X, in the inset 20X. (D) Representative histology of the skin (dorsal area) of adult (12-week old) mice. Tissue sections were stained with hematoxylin and eosin. Magnification: 10X.
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