Page 141 - 2018_12-Haematologica-web

CDK9 as a potential molecular target in NK cell leukemia/lymphoma
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Figure 5. Significant therapeutic effect of BAY 1143572 in ANKL cell-bearing NOG mice. (A) Flow cytometry of mouse blood cells. Human CD45-positive cells among the mouse cells are indicated in each panel by a red square (upper panels) and are plotted to show CD16/56 and CD19 expression; ANKL cells are CD16/56-positive and CD19-negative as indicated by a red square (lower panels). The percentages of ANKL cells in whole blood of control and BAY 1142572-treated NOG mice are 28.5%, 30.9% (mean, median), and 0.4%, 0.4%, respectively. (B) Flow cytometry of mouse bone marrow cells. Human CD45-expressing cells are again indicated in each panel by red squares (upper panels) and plotted to show CD16/56 and CD19 expression; ANKL cells are CD16/56-positive and CD19-negative as indicated by red squares (lower panels). The percentages of ANKL cells in the bone marrow of control and BAY 1142572-treated NOG mice are 20.8%, 18.3% (mean, median), and 0.2%, 0.2%, respectively. (C) Flow cytometry of mouse liver cell suspensions. Human CD45-expressing cells are indicated in each panel by red squares (upper panels) and are stained for CD16/56 and CD3 expression; ANKL cells are CD16/56-positive and CD3-negative (red squares, lower panels). The percentages of ANKL cells in the livers of control and BAY 1142572-treated NOG mice are 25.4%, 27.6% (mean, median), and 0.7%, 0.5%, respectively. (D) Flow cytometry of mouse spleen cell suspensions. As above, the human CD45-expressing cells are indicated in each panel by red squares (upper panels) and ANKL cells are CD16/56-positive and CD3-negative (red squares, lower panels). The percentages of ANKL cells in the spleens of control and BAY 1142572-treated NOG mice are 18.1%, 19.3% (mean, median), and 1.0%, 0.9%, respectively. The significance of the difference (P value) in the percentages of ANKL cells between two groups is shown in each panel (A) (B) (C), and (D).
haematologica | 2018; 103(12)
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