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Protein disulfide isomerase as a target in leukemia
Figure 6. SK053 exerts antileukemic effects and induces differentiation of primary leukemic blasts isolated from the bone marrow of patients with acute myeloid leukemia. (Left) Primary AML blasts isolated from six patients (numbered consecutively Pt 1 to Pt 6) were incubated with the indicated concentrations of SK053 for 72 h. Each day the number of dead cells was evaluated with trypan blue exclusion. Graphs present mean percentages of dead cells ± SD (n=6 experiments). *P<0.05 vs. controls, one-way ANOVA with Dunnett post-hoc test. (Right) Primary AML blasts isolated from six patients were incubated with the indicated concen- trations of SK053 for 72 h. Percentages of CD11b+ cells among live (7-AAD-) and CD33+ cells were evaluated in flow cytometry. Graphs present mean percentages of dead cells ± SD (n=3 experiments). *P<0.05 vs. controls, one-way ANOVA with Dunnett post-hoc test.
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