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M. Rijkers et al.
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Figure 3. HLA monoclonal antibodies induce FcγRIIa-dependent platelet activation. (A) CD62P exposure upon incubation with WIM8E5 and SN607D8, inhibited by pre-incubation with Syk inhibitor IV compared to control (buffer only, no HLA antibodies). (B) VWF release, measured by enzyme-linked immunosorbent assay, induced by WIM8E5 and SN607D8, inhibited by Syk inhibitor IV. (C) SPARC release in platelet supernatant induced by WIM8E5 and SN607D8, inhibited by Syk inhibitor IV. (D) Integrin αIIbb3 activation (as measured by PAC-1 binding) induced by WIM8E5 and SN607D8, inhibited by Syk inhibitor IV. (E) Agglutination induced by WIM8E5, inhibited by Syk inhibitor IV. (F) CD62P exposure induced by WIM8E5 and SN607D8, blocked by the FcγRIIa blocking antibody IV.3. (G) VWF release induced by WIM8E5 and SN607D8, inhibited by IV.3 (H) Release of SPARC in platelet supernatant inhibited by IV.3. (I) Agglutination induced by WIM8E5 and SN607D8 inhibited by pre-incubation with IV.3. (J) PAC-1 binding induced upon incubation with WIM8E5 and SN607D8 inhibited by IV.3. Data are given as mean ± SD. Paired ANOVA with the Tukey multiple comparison test. *P<0.05, **P<0.01, ***P<0.005, ****P<0.001.
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haematologica | 2018; 103(10)