Page 82 - Haematologica Vol. 109 - July 2024
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ARTICLE - Targeting TNF/IL-17/MAPK in hE2A-PBX1 zebrafish
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Figure 2. Induction of hE2A-PBX1 expression in zebrafish larvae leads to myeloid cell and hematopoietic stem cell expansion. (A) Immunofluorescence staining of Lcp1+ cells in siblings (left panel) and Tg(hsp70:E2A-PBX1-EGFP) (right panel) at 5 days post-fer- tilization (dpf). (A’) Statistical analysis of the Lcp1+ signals in panel (A). The black asterisks indicate statistical difference (Student t tests, mean ± standard error of the mean [SEM]; **P<0.01) (B) Whole mount in situ hybridization (WISH) of cebp1, lyz, mpx and mfap4 expressions in Tg(hsp70:E2A-PBX1-EGFP) (right panel) were higher than siblings (left panel) at 5 dpf. The number of lyz- DsRed+ cells (caudal hematopoietic tissue [CHT] region and Sudan Black B-positive [SB+] cells in Tg(hsp70:E2A-PBX1-EGFP) (right) was higher than siblings (left) at 5 dpf. The CHT is enlarged in the red box (original magnification ×200). (B’) Statistical analysis of the positive signals (cebp1, lyz, lyz-DsRed, mpx, SB and mfap4) in panel (B). The black asterisks indicate statistical difference
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