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Figure 4. POLN and fludarabine chemoresistance in vitro. (A) MTS viability assay of MEC-1 and MEC-2 cell lines in the absence or presence of fludarabine for a treat- ment period of 24 hours (h). The percentage of viable cells is expressed as the mean ± Standard Deviation of three independent experiments. The fludarabine EC50 value for the MEC-1 cell line was 239 mM and that for the MEC-2 cell line was 433 mM. (B) Flow cytometry cell cycle analysis of MEC-1 and MEC-2 cell lines, control and treated with fludarabine for 2 h. Delta (D) is calculated as the difference of percent of cells present in the EdU+ gate by control condition (representative of three experiments). (C) DNA combing in nanochannels. The length of replicated domains is expressed in kilobase pairs (kbp). The number of tracks measured in all con- ditions was 60. The concentration of fludarabine used was 600 mM; the duration of treatment was 4 h. Medians in the dot plot are indicated by red lines.
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