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LANDMARK PAPER IN HEMATOLOGY
Another Philadelphia story*
Jerald P. Radich
Fred Hutchinson Cancer Research Center, Seattle, WA; USA E-mail: jradich@fredhutch.org
doi:10.3324/haematol.2021.280581
*“The Philadelphia Story” is a classic 1940 American romantic comedy starring a blockbuster lineup of Katharine Hepburn, Cary Grant, and James Stewart.
TITLE AUTHORS JOURNAL
A minute chromosome in human chronic granulocytic leukemia. Nowell PC, Hungerford DA
Science 1960;132:1497.
Philadelphia, in the USA, is a city steeped in history. It was in Philadelphia that the Declaration of Independence and the US Constitution were signed. The Philly cheesesteak sandwich was born there, and Benjamin Franklin died there (presumably not from the sand- wich). In 1960, Philadelphia was also home of Drs. Peter Nowell, a pathologist at the University of Pennsylvania and David Hungerford, a graduate student at the Institute for Cancer Research.
1960 was an eventful year. In the USA, John Kennedy was elected president, and 3,500 troops were sent to Vietnam, initiating a grueling and divisive 13-year war. The Olympic Games, held in Rome, were televised for the first time. Alfred Hitchcock made the horror classic, Psycho. And tucked in the Journal Science was a modest, two paragraph, 243- word abstract that would revolutionize the field of genetics and cancer, entitled “A minute chromosome in human chronic granulocytic leukemia.”1 The piece described a recur- rent chromosomal abnormality found in seven cases of chronic granulocytic (now, myelogenous) leukemia. No other chromosome abnormalities were found in the cases, and some cells had a normal karyotype, suggesting that the new chromosome was not constitutional. The abstract con-
cludes with the understated blockbuster, “The findings sug- gest a causal relationship between the chromosomal abnor- mality observed and chronic granulocytic leukemia.”
Earlier in 1960, Nowell and Hungerford had previously reported on two of these cases in a paper that included cases of acute and chronic leukemia, in which they suggested the minute chromosome might be an altered Y. In the Science abstract, five more cases of chronic myelogenous leukemia (CML) were added and, remarkably, each had the altered chromosome, making this the first demonstration of a con- sistent chromosomal abnormality found in a specific type of cancer. The rival cytogenetic research group in Edinburgh proposed that the chromosome be dubbed “the Philadelphia chromosome.”
In Peter Nowell’s own underplayed words, “the next decade was frustrating.” Further identification of the chromosome awaited the advent of new banding techniques in the 1970s pioneered by Janet Rowley, which demonstrated that the Philadelphia chromosome was actually a reciprocal translo- cation between chromosomes 22 and 9.2 Jump another decade into the 1980s, and the story is further detailed with the discovery that the translocation fused the BCR region from chromosome 22 with the human homologue of the
Figure 1. The Philadelphia chromosome. The left panel is the first image of the Philadelphia chromosome from Nowell and Hungerford’s first paper.5 This was initially thought to be a Y chromosome. The right panel shows the breakpoints involving the BCR gene from chromosome 22, and the ABL gene from chromosome 9. The bottom right panels show the fusion mRNA breakpoints found in leukemia.
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haematologica | 2022; 107(3