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CLL cells affect osteoblastogenesis/osteoclastogenesis
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Figure 3. Enhancement of osteoclast differentiation following the addition of CLL conditioned media to cultures of monocytes, from healthy donors or CLL patients, pre-stimulated with MCSF+RANKL. (A) The addition of CLL conditioned medium (cm) enhances osteoclast differentiation of healthy monocytes pre-stimulated with MCSF and RANKL, as shown here with conditioned medium from a representative case (cm FW16). The number of osteoclasts was determined by counting the num- ber of TRAP+ cells which were at least tri-nucleated. (B) Osteoclasts generated with the addition of CLL-cm show a typical morphology of multinucleated (N) large cells with a ruffle border (RF). (C) The addition of CLL-cm derived from cultures of CLL cells from different patients (BE08, CRO7, JF1h2, FW16, BE117, MG37, VG34) to monocytes purified from three healthy donors (1310, 1363, 1610) significantly increased the differentiation of monocytes, previously stimulated with MCSF+RANKL, to osteoclasts. (D, E) CLL-cm from CLL patient SD36 was added to autologous monocytes, previously stimulated with MCSF+RANKL: also in this auto- logous setting, a significant enhancement of osteoclast differentiation was observable. For the images magnified 10X, 20X or 40X, the bars represent 25, 12.5 or 6.25 mm, respectively.
haematologica | 2021; 106(10)
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