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G. Zuchtriegel et al.
from VN-/- donor mice and adoptively transferred into WT recipient mice was not significantly altered as compared to the number of accumulated fluorescence-labeled neu- trophils isolated from WT donor mice and adoptively transferred into WT mice. In contrast, the number of accu-
A
mulated fluorescence-labeled neutrophils isolated from WT mice and adoptively transferred into VN-/- recipient mice was significantly reduced as compared to the num- ber of accumulated fluorescence-labeled neutrophils iso- lated from WT donor mice and adoptively transferred into
B
Figure 2. Role of vitronectin for interactions of neutrophils and endothelial cells. (A) Using multi-channel in vivo microscopy on the inflamed mouse cremaster mus- cle, interactions of Ly-6G+ neutrophils (red) with endothelial cells were analyzed in postcapillary venules, representative still images are shown (scale bar: 20 mm). Panels show quantitative results for rolling flux, rolling velocity, short adhesion, firm adherence, adhesion time, and transmigration of neutrophils in wild-type (WT), vitronectin (VN)+/-, or VN+/+ mice (mean±standard error of the mean [SEM] for n=4 animals per group; *P<0.05 vs. WT). See also Online Supplementary Table S1. (B) Accumulation of calcein AM-labeled bone marrow (BM) leukocytes were quantified in the postischemic cremaster muscle using multi-channel in vivo fluorescence microscopy. Panel shows results for WT recipient mice receiving leukocytes from WT or VN-deficient donors as well as for VN-deficient recipient mice receiving leuko- cytes from WT donors (mean±SEM for n=5 animals per group; *P<0.05 vs. WT). n: number; s: seconds; HPF: hydroxyphenyl fluorescein; I/R: ischemia-reperfusion.
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haematologica | 2021; 106(10)