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Letters to the Editor
Helsinki Declaration. All patients provided written informed consent.
Targeted next-generation sequencing of diagnostic samples was performed using a custom-designed multi- plex Ion Ampliseq panel (Thermo Fischer Scientific, Waltham, MA, USA) including 20 genes recurrently mutated in MDS (Online Supplementary Appemdix). SNP-
A was performed on diagnostic samples using the Infinium CytoSNP-850K v1.1 BeadChip (Illumina, San Diego, CA, USA). Illumina intensity files (.idat files) were analyzed visually with the GenomeStudio software ver- sion 2011.1 (Illumina) (Online Supplementary Appemdix).
Patient characteristics at baseline are presented in the Online Supplementary Table S1 and the Online
A
B
C
Figure 1. Single nucleotide poly- morphism-based array analysis- detected structural aberrations demarcate survival in patients with idiopathic cytopenia of unde- termined significance but are not an independent adverse prognos- tic factor. Kaplan-Meier estimates of (A) overall survival and (B) pro- gression-free survival of the group of idiopathic cytopenia of undeter- mined significance (ICUS) patients with copy number aberrations (CNA) or copy neutral loss of het- erozygosity (CNLOH) (excluding loss of the Y chromosome; red curve) and the group of ICUS patients without CNA or CNLOH (black curve). (C) Forest plot of hazard ratios (HR) including 95% Confidence intervals (CI) for all- cause mortality in multivariable analysis in ICUS patients (n=109 with complete data). Overall sur- vival was measured from first bone marrow investigation (=inclusion) to death from any cause. Progression-free survival was measured from first bone marrow investigation to progression to a myeloid cancer or death from any cause. Annotated P-values are from two-sided log-rank tests. Severe anemia was defined as hemoglobin <100 g/L. Mutations were identified by targeted next generation sequencing using a 20- gene panel with a lower limit of detection at 2%.
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