Page 76 - 2021_05-Haematologica-web

A. D’Alessandro et al.
that variables such as donor sex, age, ethnicity and storage additives impacted oxidative hemolysis, with blood from female, over 60 years old, and southern Asian donors whose leucoreduction RBC were stored in additive addtive AS-3 having the lowest oxidative hemolysis, independently
of storage duration (Online Supplementary Figure S3). In this view, we elaborated the metabolomics data as a function of these variables and independently of storage duration, while controlling for potential mixed effects of combined variables (Figure 4-5; Online Supplementary Figure S4-5).
A
BC
F
DE
Figure 5. Oxidative hemolysis as a function of donor age (buckets in principal components analysis [PCA] indicate increments of age by decade across second prin- cipal component [PC2]). (A). Oxidative hemolysis and reduced glutathione (GSH - donor under 30 years of age vs. over 60 – B-D, respectively; breakdown by decades – C-E) are higher and lower, respectively in red blood cells (RBS) from donors younger than 30 at any given storage day. In F, RBC from donors over 60 vs. under 30 were not significantly different with respect to storage age or donor sex, though the number of RBC from older donors that were stored in AS-1 was significantly higher. Even when controlling for storage additive (AS-3 only - G), RBC from young donors have altered glycolysis, lower levels of pentose phosphate pathway metabo- lites, altered methionine and citrulline metabolism (samples are plotted with no distinction of storage days). 5-OXO: 5-oxoproline; 6PG: 6-phosphogluconate; CITR: citrate; DPG: 2,3-diphosphoglycerate; E4P: erythrose phosphate; FBP: fructose bisphosphate; GA3P: glyceraldehyde 3-phosphate; GLUC: glucose; GSH: reduced glu- tathione; LAC: lactate; METH: methionine; NS: not-significant; PEP: phosphoenolpyruvate; PGLY: phosphoglycerate; SAM: S-adenosylmethionine.
1296
haematologica | 2021; 106(5)